Human Serine/threonine-protein kinase PAK 3 (PAK3) ELISA Kit | AE28753HU

Human Serine/threonine-protein kinase PAK 3 (PAK3) ELISA Kit | AE28753HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PAK3

Alternative Name: RP5-914P14.3; CDKN1A; MRX30; MRX47; OPHN3; PAK3beta; bPAK; hPAK3; OTTHUMP00000023856|beta-PAK|oligophrenin-3|p21 (CDKN1A) -activated kinase 3|p21-activated kinase 3|serine/threonine-protein kinase PA

Application: ELISA

Range: 23.44-1500 pg/mL

Sensitivity: 5.86 pg/mL

Intra-Assay: ≤4.9%

Inter-Assay: ≤7.4%

Recovery: 0, 86

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PAK3 in samples. An antibody specific for PAK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: PAK3 (p21-activated kinase 2, beta-PAK) is one of three members of Group I PAK family of evolutionary conserved serine/threonine kinases.PAK3 is preferentially expressed in neuronal cells and involved in synapse formation and plasticity and mental retardation.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .