Human Serpin B6 (SERPINB6) ELISA Kit | AE20017HU

Human Serpin B6 (SERPINB6) ELISA Kit | AE20017HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: SERPINB6

Alternative Name: RP1-90J20.6; CAP; DKFZp686I04222; MGC111370; MSTP057; PI6; PTI; SPI3; OTTHUMP00000017778|OTTHUMP00000017779|OTTHUMP00000017781|OTTHUMP00000017782|protease inhibitor 6 (placental thrombin inhibitor) |

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.059 ng/mL

Intra-Assay: ≤6.3%

Inter-Assay: ≤8.6%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SERPINB6 in samples. An antibody specific for SERPINB6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINB6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: PTI is a member of an emerging class of intracellular serpins.On the basis of its interaction with thrombin, through which it was discovered, the inhibitor was operationally named the placental thrombin inhibitor (PTI) . Amino acid sequence comparisons suggested that its reactive center is located at arg341 and cys342, that it lacks a classic N-terminal signal sequence, and that it has the highest degree of similarity to intracellular serine proteinase inhibitors (serpins), such as the human monocyte/neutrophil elastase inhibitor (ELANH2) . Analysis of human tissue mRNA indicated that PTI is expressed widely, with the highest levels in cardiac and skeletal muscle and placenta.Spi3, is developmentally regulated, and that, unlike the situation in the human, Spi3 is absent from the mouse placenta.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .