Human Serum amyloid A-4 protein (SAA4) ELISA Kit | AE20835HU

Human Serum amyloid A-4 protein (SAA4) ELISA Kit | AE20835HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: SAA4

Alternative Name: C-SAA; CSAA;

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.063 ng/mL

Intra-Assay: ≤6.1%

Inter-Assay: ≤8.2%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SAA4 in samples. An antibody specific for SAA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySAA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SAA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SAA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Studying the protein and the cDNA, Whitehead et al. (1992) identified a new member of the serum amyloid A protein superfamily, designated SAA4, as constitutive apolipoprotein of high density lipoprotein. Watson et al. (1992) analyzed the structure of the gene and the derived protein structure. Steel et al. (1993) demonstrated that the SAA4 gene is located 9 kb downstream of the SAA2 gene (104751) on chromosome 11. Furthermore, it shares the same 5-prime to 3-prime orientation and has the characteristic 4-exon structure of the other members of the SAA superfamily. By fluorescence in situ hybridization using a cosmid probe carrying the SAA2 and SAA4 genes, Sellar et al. (1994) localized these genes to 11p15.1. De Beer et al. (1996) found that in the mouse the Saa4 gene is linked to the serum amyloid A gene family on chromosome 7.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .