Human sFLT (Soluble Fms-Like Tyrosine Kinase Receptor 1) ELISA Kit | ELK9339

Human sFLT (Soluble Fms-Like Tyrosine Kinase Receptor 1) ELISA Kit | ELK9339 | Gentaur UK, US & Europe Distribution

Alternative Names: Flt1; Fms-Related Tyrosine Kinase 1; Vascular Permeability Factor Receptor; Fms-like tyrosine kinase 1; Tyrosine-protein kinase receptor FLT

Species: Human

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates, saliva, cell lysates, follicular fluid and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Soluble Fms-Like Tyrosine Kinase Receptor 1 (sFLT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Soluble Fms-Like Tyrosine Kinase Receptor 1 (sFLT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Soluble Fms-Like Tyrosine Kinase Receptor 1 (sFLT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Soluble Fms-Like Tyrosine Kinase Receptor 1 (sFLT) in the samples is then determined by comparing the OD of the samples to the standard curve.