Human SKP2 (S-Phase Kinase Associated Protein 2) ELISA Kit | ELK4585

Human SKP2 (S-Phase Kinase Associated Protein 2) ELISA Kit | ELK4585 | Gentaur UK, US & Europe Distribution

Alternative Names: FBL1; FBXL1; FLB1; p45; Cyclin-A/CDK2-associated protein p45; F-box/LRR-repeat protein 1; p45skp2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S-Phase Kinase Associated Protein 2 (SKP2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to S-Phase Kinase Associated Protein 2 (SKP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S-Phase Kinase Associated Protein 2 (SKP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S-Phase Kinase Associated Protein 2 (SKP2) in the samples is then determined by comparing the OD of the samples to the standard curve.