Human SMOC1 (SPARC related modular calcium binding 1) ELISA Kit | ELK9498

Human SMOC1 (SPARC related modular calcium binding 1) ELISA Kit | ELK9498 | Gentaur UK, US & Europe Distribution

Alternative Names: N/A

Species: Human

Assay Type: Sandwich

Sensitivity: 21.5 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, plasma, tissue homogenates

Assay Lenght: 3.5h

Research Area: eye and limb development

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SPARC related modular calcium binding 1 (SMOC1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SPARC related modular calcium binding 1 (SMOC1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SPARC related modular calcium binding 1 (SMOC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SPARC related modular calcium binding 1 (SMOC1) in the samples is then determined by comparing the OD of the samples to the standard curve.