Human Soluble Toll-like Receptor 6 (TLR-6) ELISA Kit | KTE62322

Human Soluble Toll-like Receptor 6 (TLR-6) ELISA Kit | KTE62322 | Gentaur UK, US & Europe Distribution

Application: This Human Soluble Toll-like Receptor 6 (TLR-6) ELISA Kit employs a two-site sandwich ELISA to quantitate TLR6 in samples. An antibody specific for TLR6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTLR6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Soluble Toll-like Receptor 6 microplate
• Human Soluble Toll-like Receptor 6 standard
• Human Soluble Toll-like Receptor 6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Soluble Toll-like Receptor 6 (TLR-6) ELISA Kit has high sensitivity and excellent specificity for detection of Human TLR6. No significant cross-reactivity or interference between Human TLR6 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Toll Like Receptor 6 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. By screening a human placenta library with a mouse Tlr6 probe, Takeuchi et al. (1999) isolated a cDNA encoding human TLR6. The deduced 796-amino acid type I transmembrane protein, which is 74% identical to mouse Tlr6 and 69% identical to TLR1, contains an N-terminal signal peptide, 20 tandemly repeated extracellular leucine-rich motifs, and a cytoplasmic domain homologous to IL1R, as seen in other TLRs. RT-PCR analysis detected Tlr6 in mouse thymus, spleen, ovary, and lung.

Alternative Names: TLR6; CD286; OTTHUMP00000158730

Search name: TLR6; CD286; OTTHUMP00000158730

Tag: TLR6