Human SPAG9 (Sperm Associated Antigen 9) ELISA Kit | ELK5470

Human SPAG9 (Sperm Associated Antigen 9) ELISA Kit | ELK5470 | Gentaur UK, US & Europe Distribution

Alternative Names: HSS; PIG6; HLC4; JLP; PHET; C-Jun-Amino-Terminal Kinase-Interacting Protein 4; Lung Cancer Oncogene 4; Proliferation-Inducing Gene 6; Cancer/Testis Antigen 89

Species: Human

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Reproductive science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sperm Associated Antigen 9 (SPAG9) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sperm Associated Antigen 9 (SPAG9) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sperm Associated Antigen 9 (SPAG9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sperm Associated Antigen 9 (SPAG9) in the samples is then determined by comparing the OD of the samples to the standard curve.