Human Speckle-type POZ protein-like (SPOPL) ELISA Kit | AE15017HU

Human Speckle-type POZ protein-like (SPOPL) ELISA Kit | AE15017HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: SPOPL

Alternative Name: FLJ53775; HIB homolog 2|roadkill homolog 2

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤6.1%

Inter-Assay: ≤9.3%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SPOPL in samples. An antibody specific for SPOPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPOPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOPL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOPL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: SPOPL Belongs to the Tdpoz family.Contains 1 BTB (POZ) domain.Contains 1 MATH domain.TDPOZ, a family of bipartite animal and plant proteins that contain the TRAF (TD) and POZ/BTB domains. The Tdpoz gene cluster is mapped on chromosome 3 at 3F2.1-2.2. RT-PCR experiments and a search of expressed sequence tag (EST) databases show that the Tdpoz1-5 genes are transcribed in early embryos, particularly at the two-cell stage. TDPOZ proteins form a new protein family on the basis of similar protein domain organization. TDPOZ proteins may be nuclear scaffold proteins probably involved in transcription regulation in early development and other cellular processes.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .