Human Sperm associated antigen 9 antibody (SPAG9-Ab) ELISA Kit | AE62533HU

Human Sperm associated antigen 9 antibody (SPAG9-Ab) ELISA Kit | AE62533HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: SPAG9-Ab

Alternative Name: N/A

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.054 ng/mL

Intra-Assay: ≤4.5%

Inter-Assay: ≤8.6%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Competitive ELISA

Analysis Method : Qualitative

Test Principale: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SPAG9-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated SPAG9-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled SPAG9-Ab and unlabeled SPAG9-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of SPAG9-Ab in the sample. The color development is stopped and the intensity of the color is measured.

Product Overview: Extracellular signals are transduced into cells through mitogen-activated protein kinases. The structural organization of these kinases into specific signaling domains is facilitated by scaffolding proteins involved in closely tethering different kinases so that successive phosphorylation events can occur.Sperm Associated Antigen 9 is a scaffolding protein that brings together mitogen-activated protein kinases and their transcription factor targets for the activation of specific signaling pathways. This gene which is abundantly expressed in testicular haploid germ cells encodes a protein that is recognized by sperm-agglutinating antibodies and implicated in infertility.Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .