Human Spermatogenesis-associated protein 5 (SPATA5) ELISA Kit | KTE60546

Human Spermatogenesis-associated protein 5 (SPATA5) ELISA Kit | KTE60546 | Gentaur UK, US & Europe Distribution

Application: This Human Spermatogenesis-associated protein 5 (SPATA5) ELISA Kit employs a two-site sandwich ELISA to quantitate SPATA5 in samples. An antibody specific for SPATA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPATA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPATA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPATA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Spermatogenesis-associated protein 5 microplate
• Human Spermatogenesis-associated protein 5 standard
• Human Spermatogenesis-associated protein 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Spermatogenesis-associated protein 5 (SPATA5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPATA5. No significant cross-reactivity or interference between Human SPATA5 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: SPATA5 encodes a member of the ATPase associated with diverse activities family, whose members are defined by a highly conserved ATPase domain. Members of this family participate in diverse cellular processes that include membrane fusion, DNA replication, microtubule severing, and protein degradation. The protein encoded by this gene has a putative mitochondrial targeting sequence and has been proposed to function in maintenance of mitochondrial function and integrity during mouse spermatogenesis. Allelic variants in SPATA5 have been associated with epilepsy, hearing loss, and mental retardation syndrome. Alternative splicing results in multiple transcript variants.

Alternative Names: SPATA5; AFG2; SPAF; ATPase family gene 2 homolog; spermatogenesis associated factor SPAF

Search name: SPATA5; AFG2; SPAF; ATPase family gene 2 homolog; spermatogenesis associated factor SPAF

Tag: SPATA5