Human SPINK1 (Serine Peptidase Inhibitor Kazal Type 1) ELISA Kit | ELK3201

Human SPINK1 (Serine Peptidase Inhibitor Kazal Type 1) ELISA Kit | ELK3201 | Gentaur UK, US & Europe Distribution

Alternative Names: PCTT; PSTI; TATI; Tumor-Associated Trypsin Inhibitor; Pancreatic secretory trypsin inhibitor; Tumor-associated trypsin inhibitor

Species: Human

Assay Type: Sandwich

Sensitivity: 0.63 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Peptidase Inhibitor Kazal Type 1 (SPINK1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Serine Peptidase Inhibitor Kazal Type 1 (SPINK1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Peptidase Inhibitor Kazal Type 1 (SPINK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine Peptidase Inhibitor Kazal Type 1 (SPINK1) in the samples is then determined by comparing the OD of the samples to the standard curve.