Human Stanniocalcin-2 (STC2) ELISA Kit | AE15580HU

Human Stanniocalcin-2 (STC2) ELISA Kit | AE15580HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: STC2

Alternative Name: STC-2; STCRP; STC-related protein|stanniocalcin-related protein

Application: ELISA

Range: 31.25-2000 pg/mL

Sensitivity: 13.6 pg/mL

Intra-Assay: ≤4.7%

Inter-Assay: ≤6.9%

Recovery: 1

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate STC2 in samples. An antibody specific for STC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Stanniocalcin-2 is a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .