Human Steroidogenic acute regulatory protein, mitochondrial (STAR) ELISA Kit | KTE60374

Human Steroidogenic acute regulatory protein, mitochondrial (STAR) ELISA Kit | KTE60374 | Gentaur UK, US & Europe Distribution

Application: This Human Steroidogenic acute regulatory protein, mitochondrial (STAR) ELISA Kit employs a two-site sandwich ELISA to quantitate STAR in samples. An antibody specific for STAR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTAR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STAR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STAR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Steroidogenic acute regulatory protein, mitochondrial microplate
• Human Steroidogenic acute regulatory protein, mitochondrial standard
• Human Steroidogenic acute regulatory protein, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Steroidogenic acute regulatory protein, mitochondrial (STAR) ELISA Kit has high sensitivity and excellent specificity for detection of Human STAR. No significant cross-reactivity or interference between Human STAR and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The steroidogenic acute regulatory protein is a transport protein that regulates cholesterol transfer within the mitochondria, which is the rate-limiting step in the production of steroid hormones. It is primarily present in steroid-producing cells, including theca cells and luteal cells in the ovary, Leydig cells in the testis and cell types in the adrenal cortex. The domain of StAR important for promoting cholesterol transfer is the StAR-related transfer domain (START domain) . StAR is the prototypic member of the START domain family of proteins and is thus also known as STARD1 for "START domain-containing protein 1". It is hypothesized that the START domain forms a pocket in StAR that binds single cholesterol molecules for delivery to P450scc.

Alternative Names: STAR; STARD1; START domain containing 1; StAR-related lipid transfer (START) domain containing 1; cholesterol trafficker; mitochondrial steroid acute regulatory protein; steroid acute regulatory protein; stero

Search name: STAR; STARD1; START domain containing 1; StAR-related lipid transfer (START) domain containing 1; cholesterol trafficker; mitochondrial steroid acute regulatory protein; steroid acute regulatory protein; stero

Tag: STAR