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Human sTWEAK (soluble tumor necrosis factor-like weak inducer of apoptosis) ELISA Kit | ELK8879
- SKU:
- 855-ELK8879-GEN
Description
Human sTWEAK (soluble tumor necrosis factor-like weak inducer of apoptosis) ELISA Kit | ELK8879 | Gentaur UK, US & Europe Distribution
Alternative Names: soluble tumor necrosis factor like weak inducer of apoptosis; sTWEAK
Species: Human
Assay Type: Sandwich
Sensitivity: 0.147 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: CD & Adhesion molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to soluble tumor necrosis factor-like weak inducer of apoptosis (sTWEAK) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to soluble tumor necrosis factor-like weak inducer of apoptosis (sTWEAK) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain soluble tumor necrosis factor-like weak inducer of apoptosis (sTWEAK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of soluble tumor necrosis factor-like weak inducer of apoptosis (sTWEAK) in the samples is then determined by comparing the OD of the samples to the standard curve.