Human Synaptotagmin-15 (SYT15) ELISA Kit | KTE60430

Human Synaptotagmin-15 (SYT15) ELISA Kit | KTE60430 | Gentaur UK, US & Europe Distribution

Application: This Human Synaptotagmin-15 (SYT15) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT15 in samples. An antibody specific for SYT15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT15 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Synaptotagmin-15 microplate
• Human Synaptotagmin-15 standard
• Human Synaptotagmin-15 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Synaptotagmin-15 (SYT15) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYT15. No significant cross-reactivity or interference between Human SYT15 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Synaptotagmin-15 (Syt15) belongs to the synaptotagmin family, which is a group of membrane-trafficking proteins that contain two C-terminal C2 domains (known as C2A and C2B domains) . Most of the synaptotagmins have a unique N-terminal domain that is involved in membrane anchoring or specific ligand binding. Mammals contain 16 synaptotagmins; eight of these bind Ca2+ via their C2-domains (Syt1-Syt3, Syt5-7, Syt9, and Syt10), whereas the other eight, including Syt15, do not.

Alternative Names: SYT15; RP11-38L15.7; CHR10SYT; sytXV; chr10 synaptotagmin; synaptotagmin XV-a

Search name: SYT15; RP11-38L15.7; CHR10SYT; sytXV; chr10 synaptotagmin; synaptotagmin XV-a

Tag: SYT15