Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4) ELISA Kit | KTE62326

Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4) ELISA Kit | KTE62326 | Gentaur UK, US & Europe Distribution

Application: This Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4) ELISA Kit employs a two-site sandwich ELISA to quantitate TIMD4 in samples. An antibody specific for TIMD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIMD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMD4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human T-cell immunoglobulin and mucin domain-containing protein 4 microplate
• Human T-cell immunoglobulin and mucin domain-containing protein 4 standard
• Human T-cell immunoglobulin and mucin domain-containing protein 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4) ELISA Kit has high sensitivity and excellent specificity for detection of Human TIMD4. No significant cross-reactivity or interference between Human TIMD4 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: TIM4 has an N-terminal IgV domain, followed by a long mucin stalk and a C-terminal cytoplasmic tail. The IgV domain contains an N-linked glycosylation site and a potential integrin-binding RGD motif, and the mucin stalk contains 38 potential O-linked glycosylation sites and a second N-linked glycosylation site near the membrane. The cytoplasmic domain of TIM4 lacks a conserved tyrosine kinase phosphorylation site found in other TIM family members. The antigen recognized by the antibody was identified by expression cloning as a type I transmembrane protein called Tim4. Tim4 was expressed in Mac1+ cells in various mouse tissues, including spleen, lymph nodes, and fetal liver. Tim4 bound apoptotic cells by recognizing phosphatidylserine via its immunoglobulin domain.

Alternative Names: TIMD4; FLJ27515; SMUCKLER; TIM4;

Search name: TIMD4; FLJ27515; SMUCKLER; TIM4;

Tag: TIMD4