Human T-lymphoma invasion and metastasis-inducing protein 1 (TIAM1) ELISA Kit | KTE62328

Human T-lymphoma invasion and metastasis-inducing protein 1 (TIAM1) ELISA Kit | KTE62328 | Gentaur UK, US & Europe Distribution

Application: This Human T-lymphoma invasion and metastasis-inducing protein 1 (TIAM1) ELISA Kit employs a two-site sandwich ELISA to quantitate TIAM1 in samples. An antibody specific for TIAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIAM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human T-lymphoma invasion and metastasis-inducing protein 1 microplate
• Human T-lymphoma invasion and metastasis-inducing protein 1 standard
• Human T-lymphoma invasion and metastasis-inducing protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human T-lymphoma invasion and metastasis-inducing protein 1 (TIAM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TIAM1. No significant cross-reactivity or interference between Human TIAM1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: TRIF is primarily active in the spleen and is often regulated when MyD88 is deficient in the liver, indicating organ-specific regulation of signaling pathways. Curiously, there is a lack of redundancy within the TLR4 signaling pathway that leads to microbial evasion of immune response in the host after mutations occur within intermediates of the pathway. Three TRAF-binding motifs present in the amino terminal region of TRIF are necessary for association with TRAF6. Destruction of these motifs reduced the activation of NF-κβ, a transcription factor that is also activated by the carboxy-terminal domain of TRIF in the upregulation of cytokines and co-stimulatory immune molecules. This domain recruits receptor-interacting protein (RIP1) and RIP3 through the RIP homotypic interaction motif.

Alternative Names: TIAM1; FLJ36302; human T-lymphoma invasion and metastasis inducing TIAM1 protein

Search name: TIAM1; FLJ36302; human T-lymphoma invasion and metastasis inducing TIAM1 protein

Tag: TIAM1