Human Tankyrase-1 (TNKS) ELISA Kit | AE13946HU

Human Tankyrase-1 (TNKS) ELISA Kit | AE13946HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: TNKS

Alternative Name: PARP-5a; PARP5A; PARPL; TIN1; TINF1; TNKS1;

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤4.1%

Inter-Assay: ≤7.6%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate TNKS in samples. An antibody specific for TNKS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNKS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNKS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNKS bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Tankyrase 1, a human telomeric poly (ADP-ribose) polymerase, was originally identified through its interaction with TRF1, a negative regulator of telomere length. Tankyrase 1 ADP-ribosylates TRF1 in vitro, and its overexpression induces telomere elongation in human cancer cells. Surprisingly, dissection of this domain reveals multiple discrete and overlapping binding sites for TRF1 and TAB182. Five well conserved ankyrin repeat clusters in tankyrase 1. Although each of the five ankyrin repeat clusters independently binds to TRF1, only three of the five bind toTAB182. These findings suggest that tankyrase 1 may act as a scaffold for large molecular mass complexes made up of multiple binding proteins. Potential roles for tankyrase 1-mediated higher order complexes at telomeres and at other subcellular sites.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .