Human TCN2 (Transcobalamin II, Macrocytic Anemia) ELISA Kit | ELK3759

Human TCN2 (Transcobalamin II, Macrocytic Anemia) ELISA Kit | ELK3759 | Gentaur UK, US & Europe Distribution

Alternative Names: TC2; Transcobalamin II

Species: Human

Assay Type: Sandwich

Sensitivity: 0.065 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, saliva, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcobalamin II, Macrocytic Anemia (TCN2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transcobalamin II, Macrocytic Anemia (TCN2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcobalamin II, Macrocytic Anemia (TCN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transcobalamin II, Macrocytic Anemia (TCN2) in the samples is then determined by comparing the OD of the samples to the standard curve.