Human TEX101 (testis expressed 101) ELISA Kit | ELK9066

Human TEX101 (testis expressed 101) ELISA Kit | ELK9066 | Gentaur UK, US & Europe Distribution

Alternative Names: Cell surface receptor NYD SP8; GTPR867; NYD SP8; PRO1884; SGRG; TES101RP; testis expressed 101; Testis expressed protein 101; TEX101

Species: Human

Assay Type: Sandwich

Sensitivity: 0.294 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Reproductive science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to testis expressed 101 (TEX101) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to testis expressed 101 (TEX101) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain testis expressed 101 (TEX101), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of testis expressed 101 (TEX101) in the samples is then determined by comparing the OD of the samples to the standard curve.