Human TGF-beta-inducible early response gene-1 (TIEG1) ELISA Kit | KTE61907

Human TGF-beta-inducible early response gene-1 (TIEG1) ELISA Kit | KTE61907 | Gentaur UK, US & Europe Distribution

Application: This Human TGF-beta-inducible early response gene-1 (TIEG1) ELISA Kit employs a two-site sandwich ELISA to quantitate KLF10 in samples. An antibody specific for KLF10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLF10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLF10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLF10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human TGF-beta-inducible early response gene-1 microplate
• Human TGF-beta-inducible early response gene-1 standard
• Human TGF-beta-inducible early response gene-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human TGF-beta-inducible early response gene-1 (TIEG1) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLF10. No significant cross-reactivity or interference between Human KLF10 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Tachibana et al. characterized the expression and function in exocrine pancreatic epithelial cells of the TGF-beta-inducible early gene (TIEG), a Kruppel-like zinc finger transcription factor-encoding gene previously isolated from mesodermally derived osteoblastic cells by Subramaniam et al. Tachibana et al. demonstrated that the gene is expressed in both acinar and ductular epithelial cell populations from the exocrine pancreas. In addition, they showed that the expression of TIEG is regulated by TGF-beta-1 (TGFB1) as an early response gene in pancreatic epithelial cell lines. Overexpression of TIEG in a TGF-beta-sensitive epithelial cell line was sufficient to induce apoptosis. Thus, Tachibana et al. concluded that TIEG plays a role in linking TGF-beta-mediated signaling cascades to the regulation of pancreatic epithelial cell growth.

Alternative Names: KLF10; EGRA; TIEG; TIEG1; TGFB inducible early growth response

Search name: KLF10; EGRA; TIEG; TIEG1; TGFB inducible early growth response

Tag: KLF10