Human Thymosin beta-4, Y-chromosomal (TMSB4Y) ELISA Kit | AE59578HU

Human Thymosin beta-4, Y-chromosomal (TMSB4Y) ELISA Kit | AE59578HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: TMSB4Y

Alternative Name: MGC26307; TB4Y; thymosin beta-4; Y isoform|thymosin; beta 4; Y chromosome

Application: ELISA

Range: 123.46-10000 ng/mL

Sensitivity: 42.2 ng/mL

Intra-Assay: ≤5.3%

Inter-Assay: ≤10.2%

Recovery: 1, 02

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate TMSB4Y in samples. An antibody specific for TMSB4Y has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMSB4Y present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMSB4Y is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMSB4Y bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Lahn and Page (1997) isolated testis cDNAs encoding TB4Y, a Y-linked TB4X homolog. They identified TB4Y as 1 of 5 NRY genes that were expressed in many tissues and have homologs on the X chromosome that escape X inactivation. The antigen, an 11-mer peptide (EVLLRPGLHFR), was encoded by a polymorphic region 405 bp upstream of the initiation codon of TMSB4Y. CTL precursor frequency analysis showed that approximately 25% of the total donor CTL responses in the patient 50 days after transplant were directed at this epitope, and the precursor was detectable almost 2 years after transplant. RT-PCR analysis detected wide expression of TMSB4Y mRNA in normal tissues, including hematopoietic cells, and TMSB4Y was also expressed in some leukemic cells.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .