Human TIEG1 (TGF Beta Inducible Early Response Gene 1) ELISA Kit | ELK1827

Human TIEG1 (TGF Beta Inducible Early Response Gene 1) ELISA Kit | ELK1827 | Gentaur UK, US & Europe Distribution

Alternative Names: KLF10; EGRA; TIEG; Krueppel-Like Factor 10; TGFB Inducible Early Growth Response; EGR-alpha; Transforming growth factor-beta-inducible early growth response protein 1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.124 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Apoptosis;Tumor immunity;Infection immunity;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF Beta Inducible Early Response Gene 1 (TIEG1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TGF Beta Inducible Early Response Gene 1 (TIEG1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TGF Beta Inducible Early Response Gene 1 (TIEG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TGF Beta Inducible Early Response Gene 1 (TIEG1) in the samples is then determined by comparing the OD of the samples to the standard curve.