Human TMPRSS4 (Transmembrane Protease, Serine 4) ELISA Kit | ELK3730

Human TMPRSS4 (Transmembrane Protease, Serine 4) ELISA Kit | ELK3730 | Gentaur UK, US & Europe Distribution

Alternative Names: MT-SP2; CAPH2; TMPRSS3; Membrane-Type Serine Protease 2; Type II Membrane Serine Protease; Channel-activating protease 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.063 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane Protease, Serine 4 (TMPRSS4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transmembrane Protease, Serine 4 (TMPRSS4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transmembrane Protease, Serine 4 (TMPRSS4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transmembrane Protease, Serine 4 (TMPRSS4) in the samples is then determined by comparing the OD of the samples to the standard curve.