Human Transfusion transimitted virus antibody (TTV-Ab) ELISA Kit | AE62573HU

Human Transfusion transimitted virus antibody (TTV-Ab) ELISA Kit | AE62573HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: TTV-Ab

Alternative Name: hepatitis H virus

Application: ELISA

Range: 0.781-50 ng/mL

Sensitivity: 0.28 ng/mL

Intra-Assay: ≤5.6%

Inter-Assay: ≤9.8%

Recovery: 0, 89

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Competitive ELISA

Analysis Method : Qualitative

Test Principale: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TTV-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated TTV-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled TTV-Ab and unlabeled TTV-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of TTV-Ab in the sample. The color development is stopped and the intensity of the color is measured.

Product Overview: Exostosin-1 is an endoplasmic reticulum-resident type II transmembrane glycosyltransferase involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type I form of multiple exostoses.By screening a human chondrocyte cDNA library with cosmids that spanned breakpoints on chromosome 8q identified in patients with multiple exostoses type I, Ahn et al. (1995) identified a cDNA encoding a putative 746-amino acid protein with a molecular mass of 86.3 kD. Northern blot analysis detected expression of a 3.4-kb transcript in all tissues tested, with highest levels in liver. The authors noted that the breakpoint region in the EXT1 gene contains 2 identical polypyrimidine tracts (CCCCCCT) that are known to be deletion hotspots, similar to the retinoblastoma gene.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .