Human Translocating chain-associated membrane protein 1 (TRAM1) ELISA Kit | AE13543HU

Human Translocating chain-associated membrane protein 1 (TRAM1) ELISA Kit | AE13543HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: TRAM1

Alternative Name: PNAS8; PRO1292; TRAM; TRAMP; translocating chain-associating membrane protein|translocation-associating membrane protein 1

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.059 ng/mL

Intra-Assay: ≤4.1%

Inter-Assay: ≤6.9%

Recovery: 0, 8

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate TRAM1 in samples. An antibody specific for TRAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRAM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: TRAM1 is a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause. TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95% amino acid identity with the canine protein. TRAM contains a potential N-terminal N-linked glycosylation site and a C-terminal 60-residue cytosolic portion. Immunoblot analysis showed that TRAM is an abundantly expressed, approximately 36-kD microsomal glycoprotein. Functional analysis indicated that TRAM influences glycosylation and is stimulatory or required for the translocation of secretory proteins.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .