Human Transmembrane emp24 domain-containing protein 9 (TMED9) ELISA Kit | KTE60300

Human Transmembrane emp24 domain-containing protein 9 (TMED9) ELISA Kit | KTE60300 | Gentaur UK, US & Europe Distribution

Application: This Human Transmembrane emp24 domain-containing protein 9 (TMED9) ELISA Kit employs a two-site sandwich ELISA to quantitate TMED9 in samples. An antibody specific for TMED9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMED9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMED9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMED9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Transmembrane emp24 domain-containing protein 9 microplate
• Human Transmembrane emp24 domain-containing protein 9 standard
• Human Transmembrane emp24 domain-containing protein 9 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Transmembrane emp24 domain-containing protein 9 (TMED9) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMED9. No significant cross-reactivity or interference between Human TMED9 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: TMED9 Belongs to the EMP24/GP25L family.Contains 1 GOLD domain. Members of the p24 (p24/gp25L/emp24/Erp) family of proteins have been shown to be critical components of the coated vesicles that are involved in the transportation of cargo molecules from the endoplasmic reticulum to the Golgi complex. The p24 proteins form hetero-oligomeric complexes and are believed to function as receptors for specific secretory cargo. The identification of the GOLD domain could aid in directed investigation of the role of the p24 proteins in the secretion process. The newly detected group of GOLD-domain proteins, which might simultaneously bind membranes and other proteins, point to the existence of a novel class of adaptors that could have a role in the assembly of membrane-associated complexes or in regulating assembly of cargo into membranous vesicles.

Alternative Names: TMED9; HSGP25L2G; gp25L2 protein

Search name: TMED9; HSGP25L2G; gp25L2 protein

Tag: TMED9