Human Twisted gastrulation protein homolog 1 (TWSG1) ELISA Kit | KTE60114

Human Twisted gastrulation protein homolog 1 (TWSG1) ELISA Kit | KTE60114 | Gentaur UK, US & Europe Distribution

Application: This Human Twisted gastrulation protein homolog 1 (TWSG1) ELISA Kit employs a two-site sandwich ELISA to quantitate TWSG1 in samples. An antibody specific for TWSG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTWSG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TWSG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TWSG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Twisted gastrulation protein homolog 1 microplate
• Human Twisted gastrulation protein homolog 1 standard
• Human Twisted gastrulation protein homolog 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Twisted gastrulation protein homolog 1 (TWSG1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TWSG1. No significant cross-reactivity or interference between Human TWSG1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The cDNA encodes a protein sharing 41% amino acid identity with Drosophila Tsg, 89% identity with the partial human Tsg sequence, and 94% identity with a mouse EST. The Tsg sequence contains a signal peptide, as expected for a secreted protein, and 2 conserved domains containing multiple cysteines at its amino and carboxy termini. At the late gastrula stage, maternal transcripts decrease and zygotic transcripts appear, specifically in the ventral region of the embryo. Tsg has ventralizing activity and is a bone morphogenetic protein (BMP) binding protein. The N-terminal domain of Tsg is sufficient to interact with BMP4 but not with chordin. Tsg competes for binding of BMP4 with the first cysteine-rich domain of chordin (CR1) but not with full-length chordin. Endogenous Tsg antagonizes CR1 activity.

Alternative Names: TWSG1; TSG; twisted gastrulation

Search name: TWSG1; TSG; twisted gastrulation

Tag: TWSG1