Human Ubiquitin-conjugating enzyme E2 D2 (UBE2D2) ELISA Kit | KTE62599

Human Ubiquitin-conjugating enzyme E2 D2 (UBE2D2) ELISA Kit | KTE62599 | Gentaur UK, US & Europe Distribution

Application: This Human Ubiquitin-conjugating enzyme E2 D2 (UBE2D2) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2D2 in samples. An antibody specific for UBE2D2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE2D2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2D2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2D2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Ubiquitin-conjugating enzyme E2 D2 microplate
• Human Ubiquitin-conjugating enzyme E2 D2 standard
• Human Ubiquitin-conjugating enzyme E2 D2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Ubiquitin-conjugating enzyme E2 D2 (UBE2D2) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBE2D2. No significant cross-reactivity or interference between Human UBE2D2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s.Ubiquitin-conjugating enzyme E2 D2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. UBE2D2 has been shown to interact with PJA1, Baculoviral IAP repeat-containing protein 3, PJA2, UBE3A and NEDD4.

Alternative Names: UBE2D2; E2 (17) KB2; PUBC1; UBC4; UBC4/5; UBCH5B; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2 D2; ubiquitin-conjugating enzyme E2-17 kDa 2; ubiquitin-conjugating enzyme E2D 2; ubiquitin-conjugating

Search name: UBE2D2; E2 (17) KB2; PUBC1; UBC4; UBC4/5; UBCH5B; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2 D2; ubiquitin-conjugating enzyme E2-17 kDa 2; ubiquitin-conjugating enzyme E2D 2; ubiquitin-conjugating

Tag: UBE2D2