Human Ubiquitin-conjugating enzyme E2 K (UBE2K) ELISA Kit | AE12495HU

Human Ubiquitin-conjugating enzyme E2 K (UBE2K) ELISA Kit | AE12495HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: UBE2K

Alternative Name: DKFZp564C1216; DKFZp686J24237; E2-25K; HIP2; HYPG; LIG; huntingtin interacting protein 2|ubiquitin carrier protein|ubiquitin-conjugating enzyme E2-25 KDA|ubiquitin-conjugating enzyme E2K|ubiquitin-p

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.057 ng/mL

Intra-Assay: ≤4.8%

Inter-Assay: ≤7.9%

Recovery: 1

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate UBE2K in samples. An antibody specific for UBE2K has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE2K present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2K is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2K bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: HIP2 belongs to the ubiquitin-conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis. The 200-amino acid HIP2 protein sequence was identical to that of bovine ubiquitin-conjugating enzyme E2-25K. Assays of the interaction of HIP2 with huntingtin revealed that HIP2 binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. Western blot analysis detected protein bands of 25, 28, and 45 kD; the 28-kD band appeared to be selective for tissues of the central nervous system.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .