Human Ubiquitin-like modifier-activating enzyme ATG7 (ATG7) ELISA Kit | KTE62300

Human Ubiquitin-like modifier-activating enzyme ATG7 (ATG7) ELISA Kit | KTE62300 | Gentaur UK, US & Europe Distribution

Application: This Human Ubiquitin-like modifier-activating enzyme ATG7 (ATG7) ELISA Kit employs a two-site sandwich ELISA to quantitate ATG7 in samples. An antibody specific for ATG7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyATG7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATG7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATG7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Ubiquitin-like modifier-activating enzyme ATG7 microplate
• Human Ubiquitin-like modifier-activating enzyme ATG7 standard
• Human Ubiquitin-like modifier-activating enzyme ATG7 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Ubiquitin-like modifier-activating enzyme ATG7 (ATG7) ELISA Kit has high sensitivity and excellent specificity for detection of Human ATG7. No significant cross-reactivity or interference between Human ATG7 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: ATG7 was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. The deduced 703-amino acid protein contains a central putative E1-like ATP-binding site (GxGxxG), conserved charged amino acids flanking the GxGxxG motif, and a putative E1 active site with a conserved catalytic cysteine. APG7L shares similarity with the E1 enzymes UBA2 and UBA3 (UBE1C), and it shares 38% identity with yeast Apg7. EST database analysis indicated that APG7L is expressed by many diverse tissues.

Alternative Names: ATG7; APG7-LIKE; APG7L; DKFZp434N0735; GSA7; APG7 autophagy 7-like; ubiquitin activating enzyme E1-like protein

Search name: ATG7; APG7-LIKE; APG7L; DKFZp434N0735; GSA7; APG7 autophagy 7-like; ubiquitin activating enzyme E1-like protein

Tag: ATG7