Human UBQLN2 (Ubiquilin 2) ELISA Kit | ELK4157

Human UBQLN2 (Ubiquilin 2) ELISA Kit | ELK4157 | Gentaur UK, US & Europe Distribution

Alternative Names: CHAP1; CHAP1/DSK2; Dsk2; LIC-2; N4BP4; PLIC-2; PLIC2; NEDD4 Binding Protein 4; Protein linking IAP with cytoskeleton 2; Ubiquitin-like product Chap1/Dsk2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.64 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquilin 2 (UBQLN2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ubiquilin 2 (UBQLN2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquilin 2 (UBQLN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquilin 2 (UBQLN2) in the samples is then determined by comparing the OD of the samples to the standard curve.