Human Uracil-DNA glycosylase (UNG) ELISA Kit | KTE60087

Human Uracil-DNA glycosylase (UNG) ELISA Kit | KTE60087 | Gentaur UK, US & Europe Distribution

Application: This Human Uracil-DNA glycosylase (UNG) ELISA Kit employs a two-site sandwich ELISA to quantitate UNG in samples. An antibody specific for UNG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUNG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UNG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UNG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Uracil-DNA glycosylase microplate
• Human Uracil-DNA glycosylase standard
• Human Uracil-DNA glycosylase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Uracil-DNA glycosylase (UNG) ELISA Kit has high sensitivity and excellent specificity for detection of Human UNG. No significant cross-reactivity or interference between Human UNG and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: UNG encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the nuclear (UNG2) and mitochondrial (UNG1) isoforms of UNG result from alternative splicing and the use of alternative promoters. The UNG1 and UNG2 proteins contain different N-terminal sequences, but the downstream 269 amino acids are common and include a short region that binds replication protein A and a larger, compact catalytic domain.

Alternative Names: UNG; DGU; DKFZp781L1143; HIGM4; UDG; UNG1; UNG15; UNG2; uracil-DNA glycosylase 1; uracil-DNA glycosylase 2; uracil-DNA glycosylase 2

Search name: UNG; DGU; DKFZp781L1143; HIGM4; UDG; UNG1; UNG15; UNG2; uracil-DNA glycosylase 1; uracil-DNA glycosylase 2; uracil-DNA glycosylase 2

Tag: UNG