552
Human Very Low Density Lipoportein (VLDL), 50% glycerol | ABMC-P09
- SKU:
- 552-P09-GEN
- Availability:
- IN STOCK
Description
Human Very Low Density Lipoportein (VLDL), 50% glycerol |P09
| Concentration: | 1 mg / ml, determined by the Lowry method |
| Source: | From fresh human plasma that has tested negative for Hepatitis C, HIV-I and HIV-II antibodies as well as Hepatitis surface antigens. |
| Purification: | After series ultracentrifugations, Very Low Density Lipoprotein (VLDL) is isolated from human plasma (Density 1.006). |
| Buffer: | 10 mM Tris-HCl, 0.14 M NaCl, 0.5 mM EDTA, 0.02% NaN3, pH 7.4; Preserved with 50 % glycerol. |
| Storage: | -20°C for long-term storage, 4°C for short- term storage. Aliquot to avoid repeated freezing and thawing. |
IMPORTANCE
Plasma lipoproteins classes can be defined according to the densities at which they are isolated, as high (HDL), low (LDL), intermediate (IDL), very low density lipoproteins (VLDL), and the chylomicrons. The assembly and secretion of VLDL particles takes place in liver cells. Since each VLDL particle has just one copy of apo-B100, the concentration of apo-B100 represents the number of VLDL particles. (Niu and Evans, 2011) Besides cardiovascular diseases, VLDL is associated with diabetes and Alzheimer disease. (Bjorntorp, 1990; Okuizumi et al., 1995) Study also has shown that endoplasmic reticulum stress induces hepatic steatosis via increased expression of the VLDL receptor in liver cell. (Jo et al., 2013)
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