Description
Human Viperin (Virus Inhibitory Protein) ELISA Kit | ELK6310 | Gentaur UK, US & Europe Distribution
Alternative Names: Cig5; RSAD2; vig1; Radical S-Adenosyl Methionine Domain Containing 2; Viperin, Endoplasmic Reticulum-Associated, Interferon-Inducible; Cytomegalovirus-induced gene 5
Species: Human
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Virus Inhibitory Protein (Viperin) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Virus Inhibitory Protein (Viperin) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Virus Inhibitory Protein (Viperin), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Virus Inhibitory Protein (Viperin) in the samples is then determined by comparing the OD of the samples to the standard curve.