Human WDR35 (WD Repeat Containing Domain Protein 35) ELISA Kit | ELK7590

Human WDR35 (WD Repeat Containing Domain Protein 35) ELISA Kit | ELK7590 | Gentaur UK, US & Europe Distribution

Alternative Names: IFT121; Intraflagellar transport protein 121 homolog

Species: Human

Assay Type: Sandwich

Sensitivity: 0.062 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WD Repeat Containing Domain Protein 35 (WDR35) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to WD Repeat Containing Domain Protein 35 (WDR35) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WD Repeat Containing Domain Protein 35 (WDR35), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WD Repeat Containing Domain Protein 35 (WDR35) in the samples is then determined by comparing the OD of the samples to the standard curve.