Human WNT11 (Wingless Type MMTV Integration Site Family, Member 11) ELISA Kit | ELK6636

Human WNT11 (Wingless Type MMTV Integration Site Family, Member 11) ELISA Kit | ELK6636 | Gentaur UK, US & Europe Distribution

Alternative Names: wnt-11; wnt11; WNT11; wnt11b

Species: Human

Assay Type: Sandwich

Sensitivity: 0.055 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Metabolic pathway;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 11 (WNT11) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 11 (WNT11) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 11 (WNT11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 11 (WNT11) in the samples is then determined by comparing the OD of the samples to the standard curve.