Human XPD (Xeroderma Pigmentosum, Complementation Group D) ELISA Kit | ELK5232

Human XPD (Xeroderma Pigmentosum, Complementation Group D) ELISA Kit | ELK5232 | Gentaur UK, US & Europe Distribution

Alternative Names: CXPD; BTF2 p80; EM9; TTD; ERCC2; TFIIH basal transcription factor complex 80 kDa; De Sanctis-Cacchione; Excision Repair Cross-Complementing Rodent Repair Deficiency 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Tumor immunity;Dermatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Xeroderma Pigmentosum, Complementation Group D (XPD) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Xeroderma Pigmentosum, Complementation Group D (XPD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Xeroderma Pigmentosum, Complementation Group D (XPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Xeroderma Pigmentosum, Complementation Group D (XPD) in the samples is then determined by comparing the OD of the samples to the standard curve.