855
Human XPG (Xeroderma Pigmentosum, Complementation Group G) ELISA Kit | ELK2235
- SKU:
- 855-ELK2235-GEN
Description
Human XPG (Xeroderma Pigmentosum, Complementation Group G) ELISA Kit | ELK2235 | Gentaur UK, US & Europe Distribution
Alternative Names: ERCM2; ERCC5; UVDR; XPGC; Excision Repair Cross-Complementing Rodent Repair Deficiency 5; Cockayne Syndrome; DNA repair protein complementing XP-G cells
Species: Human
Assay Type: Sandwich
Sensitivity: 0.128 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Developmental science;Dermatology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Xeroderma Pigmentosum, Complementation Group G (XPG) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Xeroderma Pigmentosum, Complementation Group G (XPG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Xeroderma Pigmentosum, Complementation Group G (XPG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Xeroderma Pigmentosum, Complementation Group G (XPG) in the samples is then determined by comparing the OD of the samples to the standard curve.