Human Zinc finger protein SNAI1 (SNAI1) ELISA Kit | KTE62329

Human Zinc finger protein SNAI1 (SNAI1) ELISA Kit | KTE62329 | Gentaur UK, US & Europe Distribution

Application: This Human Zinc finger protein SNAI1 (SNAI1) ELISA Kit employs a two-site sandwich ELISA to quantitate SNAI1 in samples. An antibody specific for SNAI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySNAI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SNAI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SNAI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Zinc finger protein SNAI1 microplate
• Human Zinc finger protein SNAI1 standard
• Human Zinc finger protein SNAI1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Zinc finger protein SNAI1 (SNAI1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SNAI1. No significant cross-reactivity or interference between Human SNAI1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: SNAI1 is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.A single transcript of 1.9 kb was detected in several human fetal tissues, with highest expression in kidney. The SNAI1 open reading frame encodes a deduced 264-amino acid protein containing 4 zinc finger motifs that show 87.1% identity to mouse Snai1.SNAI1 expression is higher in normal breast epithelial cell and stromal fibroblast cell lines than in breast cancer cell lines. They showed that SNAI1 interacts with a regulatory region near promoter I.3 of the human aromatase gene, resulting in repression of promoter I.3 and in downregulation of aromatase expression in normal breast tissue.

Alternative Names: SNAI1; SLUGH2; SNA; SNAH; dJ710H13.1; snail 1 homolog; snail 1 zinc finger protein; snail 1; zinc finger protein

Search name: SNAI1; SLUGH2; SNA; SNAH; dJ710H13.1; snail 1 homolog; snail 1 zinc finger protein; snail 1; zinc finger protein

Tag: SNAI1