Hyaluronidase Inhibitor Screening ELISA Kit | E4911-100

Hyaluronidase Inhibitor Screening ELISA Kit | E4911-100

Description: Hyaluronidases, also known as hyaluronoglucosidases (HYALs) are family of enzymes that catalyse the degradation of hyaluronic acid (HA) by cleaving its glycosidic bonds. HA is a nonsulfated glycosaminoglycan and a major component of the extracellular matrix. It is involved in many biological processes including structural support, cell migration and tissue turnover. In humans, there are six known HYALs and are present in organs including spleen, skin, eyes, liver, kidneys, uterus, placenta etc. and in body fluids including saliva, tears, blood, semen etc. They increase membrane permeability, and make tissues more readily permeable. However, HYALs have been also implicated in the progression of many solid tumor types including colorectal, bladder, prostate, breast and brain cancer. Currently there are various hyaluronidase inhibitors including heparins, chitosans, trimethoprim, indomethicin etc they may serve as anti-inflammatory, anti-aging, anti-microbial or anticancer agents. ’s Hyaluronidase Inhibitor Screening ELISA Kit is a high-throughput ELISA as compared to the traditional turbidity assay for identifying HYAL inhibitors. In this assay, HYAL and test inhibitors are pre-incubated before adding into the wells of a 96-well plate, pre-coated with biotin-HA. Biotin-HA is then detected using a specific avidin-probe measured at OD 450 nm. Samples containing HYAL inhibitor will have more biotin-HA sites available, thereby resulting in higher absorbance at 450 nm as compared to samples without the HYAL inhibitor. By using different concentrations of the HYAL inhibitor, the assay can also determine the IC50. The kit offers a simple, sensitive and high throughput assay to identify HYAL inhibitors as well as determine the IC50 of any specific HYAL inhibitor. The HYAL inhibitor, heparin is also included in the kit.

Alternate Name: HYAL Inhibitor Screening Kit HYAL Inhibitor Screening ELISA Kit Hyaluridase Inhibitor Screening Kit Hyaluronidase Inhibitor Screening Assay Hyaluronidase Inhibitor Screening ELISA

Tag Line: A high-throughput ELISA for identifying HYAL inhibitors.

Summary: ’s Hyaluronidase Inhibitor Screening ELISA Kit is a high-throughput ELISA as compared to the traditional turbidity assay for identifying HYAL inhibitors.

Detection Method: Absorbance at 450 nm

Sample Type:

Species Reactivity:

Applications: For screening of hyaluronidase inhibitors

Features & Benefits: The kit offers a simple, sensitive and high throughput assay to identify HYAL inhibitors as well as determine the IC50 of any specific HYAL inhibito