HydA | Iron-hydrogenase HydA1/HydA2 | AS09 514

HydA | Iron-hydrogenase HydA1/HydA2 | AS09 514 | Gentaur UK, US & Europe Distribution

Immunogen: Recombinant, full length Chlamydomonas reinhardtii HydA-2 Q8VZZ0

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS09 600 | Anti-HydA | Iron-hydrogenase HydA2 rabbit antibodiesCollection of antibodies to Chlamydomonas reinhardtiiAlgal protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 : 5000 (WB)

Molecular weight: 53, 7 | 48 kDa (after transit peptide is cleaved)

Confirmed Reactivity: Chlamydomonas reinhardtii

Predicted Reactivity: Ostreococcus sp.Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: HydA1 (497aa) has a calculated MW of 53.1 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP has a calculated MW of 47.5 kDa and runs according to its size at about 48 kDa.HydA2 (505aa) has a calculated MW of 53.7 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP can only be estimated, since the cleavage site is known only from in silico analysis. It has a calculated MW of 47.3 kDa and should run in the gel also according to its size.This antibody is binding recombinant HydA1/2 protein.

Background: Iron-hydrogenase HydA2 is catalyzing reversible oxidation of molecular hydrogen. In Chlamydomonas the protein is present in low levels of 1 µg/liter of culture.Synonyme: HYD1/1

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Fermentation

Research Area: Algae