Hydroxy Acid Oxidase Activity Assay Kit (Fluorometric) - K2010

K2010 | Hydroxy Acid Oxidase Activity Assay Kit (Fluorometric) DataSheet

Hydroxy Acid Oxidase (HAO) (EC 1.1.3.15), also known as glycolate oxidase is a flavin mononucleotide dependent enzyme that oxidizes alpha hydroxy acid to oxo acid. Glycolate is the primary substrate for HAO, which gets oxidized to glyoxalate. HAO contributes to the general pathway of fatty acid α-oxidation and plays an important role in ethylene glycol poisoning. It is expressed in liver, kidney and pancreas. The most well studied enzyme of this family is the plant glycolate oxidase that has an essential role in photorespiration. ’s Hydroxy Acid Oxidase Activity Kit is a simple plate-based fluorometric assay for measuring HAO activity in biological samples. HAO oxidizes hydroxy carboxylate, which is accompanied by the release of H₂O₂. H₂O₂ then oxidizes a non-fluorescent probe to a fluorescent product in the presence of enzyme mix via an enzymatic reaction. The assay can detect as low as 1 µU of HAO activity in biological samples.

Alternate Name : Hydroxy Acid Oxidase Activity Assay (Fluorometric), Hydroxy Acid Oxidase Activity Assay Kit, HAO Activity Assay Kit, HAO Activity Kit, Hydroxy Acid Oxidase Assay Kit (Fluorometric), Hydroxy Acid Oxidase Activity Assay, Hydroxy Acid Oxidase Activity Assay, Hydroxy Acid Oxidase Activity Kit (Fluorometric), HAO Assay Kit (Fluorometric), HAO Activity Kit (Fluorometric), HAO Activity Assay, HAO Activity Assay Kit (Fluorometric), Hydroxy Acid Oxidase Activity Kit, Hydroxy Acid Oxidase Activity Kit

Tag Line : for measuring HAO activity in biological samples

Summary : Hydroxy Acid Oxidase (HAO) (EC 1.1.3.15), also known as glycolate oxidase is a flavin mononucleotide dependent enzyme that oxidizes alpha hydroxy acid to oxo acid. Glycolate is the primary substrate for HAO, which gets oxidized to glyoxalate. HAO contributes to the general pathway of fatty acid α-oxidation and plays an important role in ethylene glycol poisoning. It is expressed in liver, kidney and pancreas. The most well studied enzyme of this family is the plant glycolate oxidase that has an essential role in photorespiration. ’s Hydroxy Acid Oxidase Activity Kit is a simple plate-based fluorometric assay for measuring HAO activity in biological samples. HAO oxidizes hydroxy carboxylate, which is accompanied by the release of H2O2. H2O2 then oxidizes a non-fluorescent probe to a fluorescent product in the presence of enzyme mix via an enzymatic reaction. The assay can detect as low as 1 µU of HAO activity in biological samples.

Detection Method : Fluorescence (Ex/Em = 535/587 nm)

Sample Type : Purified protein, Cell lysate, Recombinant enzyme, Plant tissue lysate, Animal tissue lysate (Liver tissue)

Species Reactivity : All

Applications : Measurement of HAO activity

Features and Benefits : The assay can detect as low as 1 µU of HAO activity in biological samples., Simple plate-based fluorometric assay for measuring HAO activity in biological samples.