Hydroxyacid Oxidase-1/HAO-1, Human Recombinant | P1474

Glycolate oxidase is a member of the superfamily of the α­hydroxy acid oxidases (HAO), enzymes that are present in both plants and animals. It catalyzes the FMN­mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. The co­factor, FMN, is tightly bound but not covalently linked to the protein. In humans and other vertebrates, HAOs are found primarily in the peroxisomes of liver, kidney, and pancreas. Three HAOs have been identified in humans. HAO­1 is most highly expressed in liver and pancreas and is most active on glycolate, two­carbon substrates. Glycolate oxidase oxidizes glycolic acid to glyoxylate, and can also oxidize glyoxylate into oxalate. These reactions are central to the toxicity of ethylene glycol poisoning. Recently, HAO has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones.

P1474 | Hydroxyacid Oxidase-1/HAO-1, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: HAOX1, Glycolate Oxidase, GOX, HAO1, GOX1

Alternates names: HAOX1, Glycolate Oxidase, GOX, HAO1, GOX1

Taglines: Member of the α-­hydroxy superfamily, oxidizes glycolic acid to glyoxylate.

NCBI Gene ID #: 54363

NCBI Gene Symbol: HAO1

Gene Source: Human

Accession #: Q9UJM8

Recombinant: True

Source: E coli

Purity by SDS-PAGE: >95%

Assay: SDS-PAGE

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): >20 mU/mg. Specific activity was measured by its ability to oxidize glyoxylate.

Biological activity:

Results:

Binding Capacity:

Unit Definition: One unit of HAO activity is the amount of enzyme that generates 1.0 umol of H2O2 per minute at pH 7.5 at 25°C.

Molecular Weight: 43 kDa (N-His Tag)

Concentration:

Appearance: Liquid

Physical form description: Liquid

Reconstitution Instructions:

Amino acid sequence: aa 1-370

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans