I kappa B Kinase Detection Set | PSI-1821

I kappa B Kinase Detection Set | PSI-1821 | Gentaur UK, US & Europe Distribution

Host: N/A

Reactivity: Human

Homology: N/A

Immunogen: Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.

Tested Application: WB, IHC, ICC, IF

Application: These polyclonal antibodies can be used for detection of IKKα, IKKβ, IKKγ and IKKε by immunoblot at 0.5 - 2 μg/mL, and by immuncytochemistry (IKKα, IKKβ and IKKγ) or immunohistochemistry (IKKε) at 1 - 10 μg/mL, and Immunoflourescence.

Specificity: N/A

Purification: Antibodies are supplied as affinity chromatography purified IgG.

Clonality: Polyclonal

Isotype: N/A

Conjugate: N/A

Physical State: Liquid

Buffer: PBS containing 0.02% sodium azide.

Concentration: Antibody 1 mg/mL

Storage Condition: Stable at 4˚C for three months, store at -20˚C for up to one year.

Background: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB kinase (IKK), which phosphorylates IκB and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact with each other and both are essential for the NF-κB activation. IKKα specifically phosphorylates IκB-α, while IKKβ phosphorylates both IκB-α and IκB-β. Another molecule in the IKK complex termed IKKγ (also known as NEMO) interacts with IKKβ and is required for the activation of IKK complex and NF-κB by LPS, PMA, TNF, and IL-1 stimulation. IKKγ was also shown to bind to RIP and NIK and to mediate TNF-induced NF-κB activation. IKKε is required for the activation of NF-κB by PMA and T cell receptors but not by TNFα and IL-1. IKKε message is expressed in a variety of tissues and is inducible by TNFα, IL-1, and LPS.

For images please see PDF data sheet

User Note: Optimal dilutions for each application to be determined by the researcher.