ICAM-3, Human Recombinant | P1564

ICAM-3/CD50, also known as CD50 (Cluster of Differentiation 50), is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulinlike C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leukocytes and maybe the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signaling molecule. Recombinant human ICAM-3/CD50, fused hIgG-His-tag at C-terminus, was expressed in the insect cell and purified by using conventional chromatography techniques.

P1564 | ICAM-3, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: ICAM3, Intercellular adhesion molecule 3, ICAM-3, CD50, CDW50, ICAM-R, intercellular adhesion molecule 3 isoform 1

Alternates names: ICAM3, Intercellular adhesion molecule 3, ICAM-3, CD50, CDW50, ICAM-R, intercellular adhesion molecule 3 isoform 1

Taglines: Contributes to apoptotic neutrophil phagocytosis by macrophages

NCBI Gene ID #: 3385

NCBI Gene Symbol: ICAM3

Gene Source: Human

Accession #: P32942

Recombinant: TRUE

Source: Baculovirus

Purity by SDS-PAGE: >95%

Assay: SDS - PAGE

Purity:

Assay #2:

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Activity (Specifications/test method):

Biological activity: Measured by the ability of the immobilized protein to support the adhesion of HL-60 human promyelocytic cells. When cells are added to Human ICAM-3/CD50 coated plates, the ED50 range ≤4ug/ml.

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: 76.7 kDa (hIgG-His-tag at C-terminus)

Concentration:

Appearance: Liquid

Physical form description: Liquid

Reconstitution Instructions:

Amino acid sequence: aa 30-485

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans