IDH1 Antibody | 5819

IDH1 Antibody | 5819 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Sheep: (100%) , Bovine: (100%)

Immunogen: IDH1 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of human IDH1.
The immunogen is located within the last 50 amino acids of IDH1.

Research Area: Homeostasis

Tested Application: E, WB

Application: IDH1 antibody can be used for detection of IDH1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1211 - HepG2 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: IDH1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: IDH1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: IDH1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: IDH1 Antibody: IDH, IDP, IDCD, IDPC, PICD, HEL-216, HEL-S-26, Cytosolic NADP-isocitrate dehydrogenase, IDH

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: IDH1 Antibody: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+) . Two NADP (+) -dependent isocitrate dehydrogenases have been found as homodimer: IDH1 is predominantly cytosolic and peroxisomal and IDH2 is mitochondrial. The presence of IDH1 in peroxisomes suggests it may play a role in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic IDH1 serves a significant role in cytoplasmic NADPH production. Defects in IDH1 are involved in the development of glioma.