IDH3B Antibody | 15-180

IDH3B Antibody | 15-180 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 35-170 of human IDH3B (NP_008830.2) .

Research Area: Cancer, Signal Transduction

Tested Application: WB, IHC, IF

Application: WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100

Specificiy: N/A

Positive Control 1: NCI-H460

Positive Control 2: HeLa

Positive Control 3: 293T

Positive Control 4: Mouse kidney

Positive Control 5: Mouse heart

Positive Control 6: Mouse lung

Molecular Weight: Observed: 42kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: isocitric dehydrogenase subunit beta, isocitrate dehydrogenase, NAD (+) -specific, mitochondrial, beta subunit, isocitrate dehydrogenase [NAD] subunit beta, NAD+-specific isocitrate dehydrogenase beta, NAD+-specific isocitrate dehydrogenase b subunit, NAD+-specific ICDH, NAD (+) -specific ICDH subunit beta, RP46, MGC903, H-IDHB, FLJ11043, isocitrate dehydrogenase 3 (NAD+) beta, IDH3B

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+) . Five isocitrate dehydrogenases have been reported: three NAD (+) -dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+) -dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD (+) -dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD (+) -dependent isocitrate dehydrogenase. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.