Description
IDH3G Antibody | 15-182 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-170 of human IDH3G (NP_004126.1) .
Research Area: Cancer, Signal Transduction
Tested Application: WB
Application: WB: 1:500 - 1:2000
Specificiy: N/A
Positive Control 1: U-87MG
Positive Control 2: A-431
Positive Control 3: HepG2
Positive Control 4: MCF-7
Positive Control 5: NIH/3T3
Positive Control 6: Mouse brain
Molecular Weight: Observed: 43kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: isocitric dehydrogenase subunit gamma, isocitrate dehydrogenase, NAD (+) -specific, mitochondrial, gamma subunit, isocitrate dehydrogenase [NAD] subunit gamma, OTTHUMP00000025988, OTTHUMP00000025987, OTTHUMP00000025984, NAD+-specific ICDH, NAD (+) -specific ICDH subunit gamma, NAD (H) -specific isocitrate dehydrogenase gamma subunit, IDH-gamma, H-IDHG, isocitrate dehydrogenase 3 (NAD+) gamma, IDH3G
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+) . Five isocitrate dehydrogenases have been reported: three NAD (+) -dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+) -dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD (+) -dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD (+) -dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined.